TY - JOUR
T1 - Rapid shoot regeneration in industrial 'high starch' sweetpotato (Ipomoea batatas L.) genotypes
AU - Santa-Maria, Monica
AU - Pecota, Kenneth V.
AU - Yencho, Craig G.
AU - Allen, George
AU - Sosinski, Bryon
N1 - Funding Information:
Acknowledgements This work was funded by Bayer CropScience Corporation, the Golden LEAF Foundation, and The Consortium for Plant Biotechnology Research. Special thanks to the ‘Micropropagation Unit’ and the ‘Plant Transformation Laboratory’ at North Carolina State University (NCSU) for providing infrastructure, materials, and helpful discussions.
PY - 2009/4
Y1 - 2009/4
N2 - Sweetpotato (Ipomoea batatas L.) is an important crop in North Carolina with annual production of 0.33 million tons, accounting for 37% of total US supply (USDA, Louisiana Farm Reporter 8(12), August 2008). To target industrial use, novel high-starch industrial-type varieties that contain more than 30% dry matter were developed by conventional breeding methods. In vitro cultures from selected genotypes were established using meristem culture. To establish regeneration procedures that could be coupled with transformation experiments, conditions for the induction of rapid shoot-organogenesis in leaf explants were compared using varying concentrations of the auxins 'NAA', 'IAA', '2,4-D', and '4-FA' either alone or in combination with zeatin riboside. Regeneration efficiencies, defined as the number of explants developing shoots out of the total number tested, were as high as 57% for the best genotypes, with a significant genotype-dependent response observed in all the hormone regimes evaluated. In all treatments, shoot regeneration was observed within 2 months. Our results led to the establishment of optimized in vitro regeneration procedures for the novel high-starch sweetpotato (SP) genotypes 'DM01-158', 'FTA94', 'FT489', and 'PDM P4' that are rapid and reliable.
AB - Sweetpotato (Ipomoea batatas L.) is an important crop in North Carolina with annual production of 0.33 million tons, accounting for 37% of total US supply (USDA, Louisiana Farm Reporter 8(12), August 2008). To target industrial use, novel high-starch industrial-type varieties that contain more than 30% dry matter were developed by conventional breeding methods. In vitro cultures from selected genotypes were established using meristem culture. To establish regeneration procedures that could be coupled with transformation experiments, conditions for the induction of rapid shoot-organogenesis in leaf explants were compared using varying concentrations of the auxins 'NAA', 'IAA', '2,4-D', and '4-FA' either alone or in combination with zeatin riboside. Regeneration efficiencies, defined as the number of explants developing shoots out of the total number tested, were as high as 57% for the best genotypes, with a significant genotype-dependent response observed in all the hormone regimes evaluated. In all treatments, shoot regeneration was observed within 2 months. Our results led to the establishment of optimized in vitro regeneration procedures for the novel high-starch sweetpotato (SP) genotypes 'DM01-158', 'FTA94', 'FT489', and 'PDM P4' that are rapid and reliable.
KW - High dry matter
KW - Meristem culture
KW - Organogenesis
KW - Storage roots
KW - Sweet potato
KW - Virus indexing
UR - http://www.scopus.com/inward/record.url?scp=61449166641&partnerID=8YFLogxK
U2 - 10.1007/s11240-009-9504-3
DO - 10.1007/s11240-009-9504-3
M3 - Article
AN - SCOPUS:61449166641
SN - 0167-6857
VL - 97
SP - 109
EP - 117
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 1
ER -