TY - JOUR
T1 - Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO
AU - Rodríguez-Zúñiga, Ursula Fabiola
AU - Cannella, David
AU - Giordano, Roberto De Campos
AU - Giordano, Raquel De Lima Camargo
AU - Jørgensen, Henning
AU - Felby, Claus
N1 - Publisher Copyright:
© The Royal Society of Chemistry 2015.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Sugarcane bagasse, corn stover, and wheat straw are among the most available resources for the production of cellulosic ethanol. For these biomasses we study the influence of pre-treatment methods on the chemical composition, as well as on the subsequent reactions of enzymatic hydrolysis and oxidation of cellulose. The applied pre-treatment methods are organosolv, hydrothermal, and alkaline. Hydrothermally pretreated wheat straw gave the highest cellulose conversion with 80% glucose yield and 0.8% oxidized cellulose products. Recent studies have shown that lignin is able to boost the activity of the cellulose oxidizing enzyme lytic polysaccharide monooxygenase (LPMO). The highest activity of LPMO was observed for the hydrothermally pretreated biomasses, which also contained the highest level of lignin. All hydrolyses were done at high dry matter levels using a commercial enzyme preparation containing hydrolytic and oxidative enzymes.
AB - Sugarcane bagasse, corn stover, and wheat straw are among the most available resources for the production of cellulosic ethanol. For these biomasses we study the influence of pre-treatment methods on the chemical composition, as well as on the subsequent reactions of enzymatic hydrolysis and oxidation of cellulose. The applied pre-treatment methods are organosolv, hydrothermal, and alkaline. Hydrothermally pretreated wheat straw gave the highest cellulose conversion with 80% glucose yield and 0.8% oxidized cellulose products. Recent studies have shown that lignin is able to boost the activity of the cellulose oxidizing enzyme lytic polysaccharide monooxygenase (LPMO). The highest activity of LPMO was observed for the hydrothermally pretreated biomasses, which also contained the highest level of lignin. All hydrolyses were done at high dry matter levels using a commercial enzyme preparation containing hydrolytic and oxidative enzymes.
UR - http://www.scopus.com/inward/record.url?scp=84929302496&partnerID=8YFLogxK
U2 - 10.1039/c4gc02179g
DO - 10.1039/c4gc02179g
M3 - Article
AN - SCOPUS:84929302496
SN - 1463-9262
VL - 17
SP - 2896
EP - 2903
JO - Green Chemistry
JF - Green Chemistry
IS - 5
ER -