Development of a Scaffold from the Cob of Zea mays L. “Choclo” to Obtain an In Vitro Bone Tissue Model

The lack of accessible in vitro 3D bone tissue models puts developing countries at a disadvantage in terms of research capacity and healthcare access. In this study, a decellularized corn cob scaffold was functionalized with GTMAC by adding quaternary ammonium groups and coating it with an alginate–gelatin hydrogel to promote mesenchymal stem cell adhesion, offering a low-cost platform for bone disease modeling. Systematic characterization of the scaffold demonstrated that decellularization reduced DNA content by over 80%. Chemical treatment affected the mechanical properties of the matrix, while the hydrogel coating and the functionalized surface of the scaffold promoted cell adhesion and morphology comparable to that observed in 3D cell cultures. MTT analysis showed a subtle reduction in metabolic signal in functionalized scaffolds, which may reflect changes in cell distribution and adhesion within the 3D matrix rather than cytotoxic effects.